English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27533/39387
Visitors : 2539106      Online Users : 35
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27022

Title: Control over Surface DNA Density on Gold Nanoparticles Allows Selective and Sensitive Detection of Mercury(II)
Authors: Chi-Wei Liu;Chih-Ching Huang;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: oligonucleotide;gold nanoparticles;thymine;mercur
Date: 2008
Issue Date: 2011-10-21T02:22:20Z
Publisher: Langmuir
Abstract: Abstract:We have developed a new highly selective and sensitive technique for the detection of Hg2+ using DNA-functionalized gold nanoparticles (Au NPs) and OliGreen. This system is the first that allows the detection of Hg2+ based on the release of DNA molecules, induced by conformational changes on Au NP surfaces, and its sensitivity is highly dependent upon surface DNA density. When Hg2+ ions interact with the thymidine units of the DNA molecules bound to the Au NPs through Au−S bonds, the conformations of these DNA derivatives change from linear to hairpin structures, causing the release of some of the DNA molecules from the surface of the Au NPs into the bulk solution to react with OliGreen. The fluorescence of OliGreen−DNA complexes increased with increasing concentration of Hg2+, and Hg2+ could be detected at concentrations as low as 25 nM. A linear correlation existed between the fluorescence intensity and the concentration of Hg2+ over the range 0.05−2.5 μM (R2 = 0.98). This simple and cost-effective probe was applied to determine the spiked Hg2+ in the pond samples; the recoveries (96−102%) suggested low matrix interference and high sensitivity.
Relation: 24(15), pp.8346–8350
URI: http://ntour.ntou.edu.tw/handle/987654321/27022
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML275View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback