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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27013

Title: Capillary Electrophoresis-Based Separation Techniques for the Analysis of Proteins
Authors: Yu-Fen Huang;Chih-Chin Huang;Cho-Chun Hu;Huan-Tsung Chang Professor
Contributors: NTOU:Institute of Bioscience and Biotechnology
Keywords: Capillary electrophoresis;Multidimensional separation;Stacking
Date: 2006-09
Issue Date: 2011-10-21T02:22:18Z
Abstract: Abstract:CE offers the advantages of high speed, great efficiency, as well as the requirement of minimum amounts of sample and buffer for the analysis of proteins. In this review, we summarize the CE-based techniques coupled with absorption, LIF, and MS detection systems for the analysis of proteins mostly within the past 5 years. The basic principle of each technique and its advantages and disadvantages for protein analysis are discussed in brief. Advanced CE techniques, including on-column concentration techniques and high-efficiency multidimensional separation techniques, for high-throughput protein profiling of complex biological samples and/or of single cells are emphasized. Although the developed techniques provide improved peak capacity, they have not become practical tools for proteomics, mainly because of poor reproducibility, low-sample lading capacity, and low throughput due to ineffective interfaces between two separation dimensions and that between separation and MS systems. In order to identify the complexities and dynamics of the proteomes expressed by cells, tissues, or organisms, techniques providing improved analytical sensitivity, throughput, and dynamic ranges are still demanded.
Relation: 27(18), pp.3503–3522
URI: http://ntour.ntou.edu.tw/handle/987654321/27013
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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