English  |  正體中文  |  简体中文  |  Items with full text/Total items : 27246/39089
Visitors : 2414651      Online Users : 72
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27007

Title: Bioconjugated Gold Nanodots and Nanoparticles for Protein Assays Based on Photoluminescence Quenching
Authors: Chih-Ching Huang;Cheng-Kang Chiang;Zong-Hong Lin;Kun-Hong Lee;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
Keywords: aptamer;gold nanoparticles;gold nanodots;luminescence;proteins;tumor marker
Date: 2008
Issue Date: 2011-10-21T02:22:17Z
Publisher: Analtical Chemistry
Abstract: Abstract:This study describes the first instance of the use of two differently sized Au nanoparticles (Au NPs), acting separately as donor and acceptor, in homogeneous photoluminescence quenching assays developed for the analysis of proteins. Introduction of a breast cancer marker protein, platelet-derived growth factor AA (PDGF AA), to a solution of 11-mercaptoundecanoic acid-protected, 2.0-nm photoluminescent Au nanodots (LAuND) led to the preparation of PDGF AA−LAuND as the donor. Thiol-derivative PDGF binding aptamers (Apt) and 13-nm spherical Au NPs were used to synthesize the Apt−QAuNP acceptor. The photoluminescence of PDGF AA−LAuND at 520 nm decreased when photoluminescence quenching occurred between Apt−QAuNP and PDGF AA−LAuND. We used the PDGF AA−LAuND/Apt−QAuNP-based molecular light switching system to analyze PDGFs and PDGF α-receptor in separate homogeneous solutions. In the presence of PDGFs, the interaction between Apt−QAuNP and PDGF AA−LAuND decreased as a result of competitive reactions between the PDGFs and Apt−QAuNP. Similarly, the interaction between Apt−QAuNP and PDGF AA−LAuND reduced as a result of competitive reactions between PDGF α-receptor and PDGF AA−LAuND. The limits of detection (LODs) for PDGF AA and PDGF α-receptor were 80 pM and 0.25 nM, respectively, resulting from a low background photoluminescence signal. When using the Apt−QAuNP as selectors for (a) the enrichment of PDGF AA and (b) the removal of matrixes possessing intense background fluorescence from cell media and urine samples, the LOD for PDGF AA decreased to 10 pM. Unlike quantum dots, the LAuND provide the advantages of biocompatibility, ease of bioconjugation, and minimal toxicity.
Relation: 80(5), pp.1497–1504
URI: http://ntour.ntou.edu.tw/handle/987654321/27007
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

Files in This Item:

File Description SizeFormat

All items in NTOUR are protected by copyright, with all rights reserved.


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback