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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27007

Title: Bioconjugated Gold Nanodots and Nanoparticles for Protein Assays Based on Photoluminescence Quenching
Authors: Chih-Ching Huang;Cheng-Kang Chiang;Zong-Hong Lin;Kun-Hong Lee;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: aptamer;gold nanoparticles;gold nanodots;luminescence;proteins;tumor marker
Date: 2008
Issue Date: 2011-10-21T02:22:17Z
Publisher: Analtical Chemistry
Abstract: Abstract:This study describes the first instance of the use of two differently sized Au nanoparticles (Au NPs), acting separately as donor and acceptor, in homogeneous photoluminescence quenching assays developed for the analysis of proteins. Introduction of a breast cancer marker protein, platelet-derived growth factor AA (PDGF AA), to a solution of 11-mercaptoundecanoic acid-protected, 2.0-nm photoluminescent Au nanodots (LAuND) led to the preparation of PDGF AA−LAuND as the donor. Thiol-derivative PDGF binding aptamers (Apt) and 13-nm spherical Au NPs were used to synthesize the Apt−QAuNP acceptor. The photoluminescence of PDGF AA−LAuND at 520 nm decreased when photoluminescence quenching occurred between Apt−QAuNP and PDGF AA−LAuND. We used the PDGF AA−LAuND/Apt−QAuNP-based molecular light switching system to analyze PDGFs and PDGF α-receptor in separate homogeneous solutions. In the presence of PDGFs, the interaction between Apt−QAuNP and PDGF AA−LAuND decreased as a result of competitive reactions between the PDGFs and Apt−QAuNP. Similarly, the interaction between Apt−QAuNP and PDGF AA−LAuND reduced as a result of competitive reactions between PDGF α-receptor and PDGF AA−LAuND. The limits of detection (LODs) for PDGF AA and PDGF α-receptor were 80 pM and 0.25 nM, respectively, resulting from a low background photoluminescence signal. When using the Apt−QAuNP as selectors for (a) the enrichment of PDGF AA and (b) the removal of matrixes possessing intense background fluorescence from cell media and urine samples, the LOD for PDGF AA decreased to 10 pM. Unlike quantum dots, the LAuND provide the advantages of biocompatibility, ease of bioconjugation, and minimal toxicity.
Relation: 80(5), pp.1497–1504
URI: http://ntour.ntou.edu.tw/handle/987654321/27007
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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