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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27005

Title: Aptamer-Modified Gold Nanoparticles for Colorimetric Determination of Platelet-Derived Growth Factors and Their Receptors
Authors: Chih-Ching Huang;Yu-Fen Huang;Zehui Cao;Weihong Tan;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
國立臺灣海洋大學:生物科技研究所
Keywords: aptamer;nanoparticle
Date: 2005
Issue Date: 2011-10-21T02:22:16Z
Publisher: Analytical Chemistry
Abstract: abstract:We have developed a highly specific sensing system for platelet-derived growth factors (PDGFs) and platelet-derived growth factor receptors (PDGFR) that uses gold nanoparticles (GNPs). We synthesized GNPs modified with an aptamer (Apt-GNPs) that is specific to PDGFs and used them to detect PDGFs by monitoring the changes in the color and extinction of the Apt-GNPs that occur as a result of aggregation. The color of the Apt-GNPs changes from red to purple at low concentrations (<400 nM), but changes only slightly at higher concentrations (>400 nM). We found that the sensitivity of the Apt-GNPs for the three PDGFs is highly salt-dependent, with an optimum condition of 200 mM NaCl. We obtained biphasic curves when plotting of the ratios of the extinction coefficients of the Apt-GNPs at 650 and 530 nm against the concentrations of PDGF-AA at various concentrations of Apt-GNPs. The linear ranges of the increases and decreases in this extinction ratio are 2.5−10 and 10−20 nM, respectively, for 0.42 nM Apt-GNPs and 25−75 and 75−200 nM, respectively, for 8.4 nM Apt-GNPs. When using 8.4 nM Apt-GNPs, the corresponding linear ranges of the increases and decreases in this extinction ratio are 15−100 and 100−400 nM, respectively, for PDGF-AB and 35−150 and 150−400 nM, respectively, for PDGF-BB. In addition, we have developed a homogeneous assay to detect the PDGF receptor-β (PDGFR-β) at concentrations as low as 3.2 nM, on the basis of the competition between the Apt-GNPs and PDGFR-β for PDGF-BB. The results we present in this paper imply that there are practical applications of Apt-GNPs in protein analysis and cancer diagnosis.
Relation: 77(17), pp.5735–5741
URI: http://ntour.ntou.edu.tw/handle/987654321/27005
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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