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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/27004

Title: Aptamer-Functionalized Gold Nanoparticles for Turn-On Light Switch Detection of Platelet-Derived Growth Factor
Authors: Chih-Ching Huang;Sheng-Hsien Chiu;Yu-Fen Huang;Huan-Tsung Chang
Contributors: NTOU:Institute of Bioscience and Biotechnology
Keywords: aptamer;gold nanoparticles;fluorescence;proteins;tumor marker
Date: 2007
Issue Date: 2011-10-21T02:22:16Z
Publisher: Analtical Chemistry
Abstract: Abstract:An aptamer modified gold nanoparticles (Apt-AuNPs) based molecular light switching sensor has been demonstrated for the analysis of breast cancer markers (platelet-derived growth factors (PDGFs) and their receptors) in homogeneous solutions. The PDGF binding aptamer has a unique structure with triple-helix conformation that allows N,N-dimethyl-2,7-diazapyrenium dication (DMDAP) and PDGF bindings. The fluorescence of DMDAP is almost completely quenched by Apt-AuNPs when it intercalates with the aptamers. Owing to high magnitudes of increases (up to 40-fold) in the turn-on fluorescence signals of DMDAP/Apt-AuNP upon PDGFs binding, the approach is highly sensitive for the detection of PDGFs. The DMDAP/Apt-AuNP probe specifically and sensitively detected PDGFs under optimal concentrations of salts and DMDAP. We also demonstrated that the Apt-AuNPs are effective selectors for enrichment of PDGF-AA from large-volume samples. The approach allows detection of PDGF-AA at a concentration down to 8 pM, showing better sensitivity than other signal aptamers. By conducting a competitive assay, we demonstrated the determination of PDGF receptor-α with LOD of 0.25 nM when using the DMDAP/Apt-AuNP as a probe.
Relation: 79(13), pp.4798–4804
URI: http://ntour.ntou.edu.tw/handle/987654321/27004
Appears in Collections:[生命科學暨生物科技學系] 期刊論文

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