National Taiwan Ocean University Institutional Repository:Item 987654321/26972
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題名: Species and sex identification of Formosa landlocked salmon using loop-mediated isothermal amplification
作者: TE-HUA HSU;YUDHA T. ADIPUTRA;HIROMI OHTA;JIN-CHYWAN GWO
貢獻者: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
關鍵詞: conservation;endangered;loop-mediated isothermal amplification (LAMP);salmonid;sex
日期: 2011-09
上傳時間: 2011-10-21T02:20:31Z
出版者: Molecular Ecology Resources
摘要: Abstract:Species and sex identification are among the most important parameters for conservation management. However, it is extremely difficult to perform such identification in Formosa landlocked salmon (Oncorhynchus masou formosanus). Both sexual dimorphism in landlocked dwarf form Formosa landlocked salmon and morphological difference among cherry salmon complex are minimal. We developed a simple, rapid and noninvasive method for identifying sex and species of this critically endangered species using a loop-mediated isothermal amplification (LAMP) technique. The LAMP assay showed the advantage of simple detection (evaluated by visual inspection), rapid reaction time (<1 h), isothermal condition (less equipment required) and high efficiency (only 0.5–5 pg of DNA was required in the reaction mixture). Therefore, the method is more economical and practical than PCR. The LAMP assay can be easily performed in the field and is a valuable tool for detecting sex ratios in wild populations and identifying species in commercial imports. This is the first application of LAMP in identifying species and sex of salmonids as far as we know and clearly shows the potential application of LAMP in molecular ecology and conservation efforts.
關聯: 11(5), pp.802-807
URI: http://ntour.ntou.edu.tw/handle/987654321/26972
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