National Taiwan Ocean University Institutional Repository:Item 987654321/26972
English  |  正體中文  |  简体中文  |  全文筆數/總筆數 : 27287/39131
造訪人次 : 2443737      線上人數 : 37
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
搜尋範圍 進階搜尋


題名: Species and sex identification of Formosa landlocked salmon using loop-mediated isothermal amplification
貢獻者: NTOU:Department of Aquaculture
關鍵詞: conservation;endangered;loop-mediated isothermal amplification (LAMP);salmonid;sex
日期: 2011-09
上傳時間: 2011-10-21T02:20:31Z
出版者: Molecular Ecology Resources
摘要: Abstract:Species and sex identification are among the most important parameters for conservation management. However, it is extremely difficult to perform such identification in Formosa landlocked salmon (Oncorhynchus masou formosanus). Both sexual dimorphism in landlocked dwarf form Formosa landlocked salmon and morphological difference among cherry salmon complex are minimal. We developed a simple, rapid and noninvasive method for identifying sex and species of this critically endangered species using a loop-mediated isothermal amplification (LAMP) technique. The LAMP assay showed the advantage of simple detection (evaluated by visual inspection), rapid reaction time (<1 h), isothermal condition (less equipment required) and high efficiency (only 0.5–5 pg of DNA was required in the reaction mixture). Therefore, the method is more economical and practical than PCR. The LAMP assay can be easily performed in the field and is a valuable tool for detecting sex ratios in wild populations and identifying species in commercial imports. This is the first application of LAMP in identifying species and sex of salmonids as far as we know and clearly shows the potential application of LAMP in molecular ecology and conservation efforts.
關聯: 11(5), pp.802-807
顯示於類別:[水產養殖學系] 期刊論文


檔案 描述 大小格式瀏覽次數



著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - 回饋