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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26846

Title: XBP-1, a key regulator of unfolded protein response, activates transcription of IGF1 and Akt phosphorylation in zebrafish embryonic cell line
Authors: Meng-Chuen Hu;Hong-Yi Gong;Gen-Hwa Lin;Shao-Yang Hu;Mark Hung-Chih Chen;Shin-Jie Huang;Ching-Fong Liao;Jen-Leih Wu
Contributors: 國立臺灣海洋大學:水產養殖學系
Keywords: XBP-1;Unfolded protein response;ER stress;IGF1;Akt;Anti-apoptosis;Zebrafish
Date: 2007-08-03
Issue Date: 2011-10-21T02:19:04Z
Publisher: Biochemical and Biophysical Research Communications
Abstract: Abstract:The unfolded protein response (UPR) is a conserved and adaptive cellular response to increase cell survival during ER stress. XBP-1 spliced form (XBP-1S) generated by IRE1 endoribonuclease is a key transcriptional regulator in UPR to activate genes involved in protein folding and degradation to restore ER function. Although Akt activation was suggested to be a pro-survival pathway activated during ER stress, the signal to trigger Akt is still not clear. In this study, we report IGF1 transcription and Akt phosphorylation are enhanced in XBP-1S stably overexpressed clone of zebrafish embryonic cell line (ZF4). In addition, zebrafish IGF1 intron1 with predicted UPRE (XBP-1S binding sites) and ERSE (ATF6/XBP-1S binding site) linked with basal promoter could be activated by XBP-1S, not by XBP-1 unspliced form (XBP-1U). Furthermore, we demonstrate that expression of endogenous IGF1 is transiently induced as XBP-1 splicing during ER stress in parallel to ER chaperone GRP78/Hspa5 and ER resided E3 ubiquitin ligase Synoviolin in ZF4 cells by quantitative PCR. Our results suggest zebrafish XBP-1S not only activates genes responsible for protein folding, transporting, glycosylation and ER associated degradation but also activates anti-apoptosis signal via IGF1/Akt pathway in unfolded protein response to cope with ER stress.
Relation: 359(3), pp.778-783
URI: http://ntour.ntou.edu.tw/handle/987654321/26846
Appears in Collections:[水產養殖學系] 期刊論文

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