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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26843

Title: Thioacetamide induced liver damage in zebrafish embryo as a disease model for steatohepatitis
Authors: Aseervatham Anusha Amali;Ravikumar Deepa Rekha;Cliff Ji-Fan Lin;Wei-Lun Wang;Hong-Yi Gong;Gour-Mour Her;Jen-Leih Wu
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: liver;steatosis;thioacetamide;zebrafish
Date: 2006-03
Issue Date: 2011-10-21T02:19:02Z
Publisher: Journal of Biomedical Science
Abstract: Abstract:Steatohepatitis has recently been increasing as a cofactor influencing the progression of fibrosis, cirrhosis, adenoma and carcinoma in liver; however, the mechanisms by which it contributes to liver injury remain uncertain. We induced steatohepatitis in zebrafish embryos using thioacetamide (TAA). TUNEL assay revealed significant increasing of apoptosis in liver after 5 days post fertilization and the increasing of apoptosis was observed to be associated with the up-regulation of apoptotic genes such as, bad, bax, P-38a, caspase-3 and 8, and JNK-1. Histological sections by oil red O stain showed the accumulation of fatty droplets which causes the pushing of the nucleus towards one side. Up-regulation of steatosis markers such as, ACC, adiponectin, PTL, CEBP- a and b , SREBP-1 was also observed. Furthermore, the elevation of glutathione peroxidase in TAA treated embryos indicated that TAA induces lipid peroxidation which leads to causes liver damage. Zebrafish has already been considered as a good human disease model and in this context; TAA-treated zebrafish may serve as a good animal model to study the molecular pathogenesis of steatohepatitis. Moreover, non-availability of specific drugs to prevent steatohepatitis, this animal model may serve as a powerful preclinical platform to study the therapeutic strategies and for evaluating chemoprevention strategies for this disease.
Relation: 13(2), pp.225-232
URI: http://ntour.ntou.edu.tw/handle/987654321/26843
Appears in Collections:[水產養殖學系] 期刊論文
[生命科學暨生物科技學系] 期刊論文

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