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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26841

Title: Thioacetamide accelerates steatohepatitis, cirrhosis and HCC by expressing HCV core protein in transgenic zebrafish Danio rerio
Authors: Ravikumar Deepa Rekha;Aseervatham Anusha Amali;Gour Mour Her;Yang Hui Yeh;Hong-Yi Gong;Shao-Yang Hu;Gen-Hwa Lin;Jen-Leih Wu
Contributors: NTOU:Department of Aquaculture
Keywords: Hepatocellular carcinoma;Thioacetamide;Zebrafish
Date: 2008-01-14
Issue Date: 2011-10-21T02:19:01Z
Publisher: Toxicology
Abstract: Abstract:Hepatocellular carcinoma (HCC) is one of the common cancers worldwide, caused by Hepatitis C virus (HCV) and hepatotoxins. Here we report the development of HCC in wild type as well as HCV core protein (HCP)-transgenic zebrafish upon treatment with a hepatotoxin, thioacetamide (TAA). Two-fold accelerated HCC development could be achieved in the TAA-treated transgenic fish, that is, the progression of the disease in TAA-treated wild type zebrafish developed HCC in 12 weeks whereas that of HCP-transgenic zebrafish shortened the HCC progression to 6 weeks. Histopathological observation showed the specific pathological features of HCC. The HCC progression was confirmed through RT-PCR that revealed an up and down regulation of different marker genes at various stages of HCC progression such as, steatohepatitis, fibrosis and HCC. Moreover, HCV core protein expressed in the HCP-transgenic zebrafish and TAA synergistically accelerate the HCC development. It must be mentioned that, this is the first report revealing HCV core protein along with TAA to induce HCC in zebrafish, particularly, in a short period of time comparing to mice model. As zebrafish has already been considered as a good human disease model and in this context, this HCC-zebrafish model may serve as a powerful preclinical platform to study the molecular events in hepatocarcinogenesis, therapeutic strategies and for evaluating chemoprevention strategies in HCC.
Relation: 243(1-2), pp.11-22
URI: http://ntour.ntou.edu.tw/handle/987654321/26841
Appears in Collections:[水產養殖學系] 期刊論文
[生命科學暨生物科技學系] 期刊論文

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