National Taiwan Ocean University Institutional Repository:Item 987654321/26817
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题名: The protective effect of chitin and chitosan against Vibrio alginolyticus in white shrimp Litopenaeus vannamei
作者: Shi-Hong Wang;Jiann-Chu Chen
贡献者: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
关键词: Litopenaeus vannamei;Vibrio alginolyticus;Chitin;Chitosan;Phenoloxidase activity;Respiratory burst;Superoxide dismutase activity;Phagocytic activity
日期: 2005-09
上传时间: 2011-10-21T02:18:52Z
出版者: Fish & Shellfish Immunology
摘要: Abstract:White shrimp, Litopenaeus vannamei, which had been injected with chitin at 4, 6 and 8 μg g−1 or chitosan at 2, 4 and 6 μg g−1, were challenged with pathogen Vibrio alginolyticus at 2×106 colony-forming units (cfu) shrimp−1 and then placed in seawater of 34‰. The survival of shrimp that received chitin or chitosan at either dose was significantly higher than that of control shrimp after 1 day, and at the termination of the experiment (6 days after the challenge). In another experiment, the total haemocyte count (THC), phenoloxidase activity, respiratory burst, superoxide dismutase (SOD) activity, and phagocytic activity to V. alginolyticus were measured when L. vannamei (10.4±0.7 g) were injected individually with chitin at 4 and 6 μg g−1 or chitosan at 2 and 4 μg g−1. L. vannamei received chitin at 6 μg g−1 or chitosan at 2 and 4 μg g−1 increased significantly its THC and respiratory burst after 2 days. L. vannamei received chitin at 6 μg g−1 or chitosan at 2 and 4 μg g−1 still maintained significantly higher phenoloxidase activity after 6 days. L. vannamei received chitin at 4 and 6 μg g−1 or chitosan at 2 and 4 μg g−1 increased its phagocytic activity against V. alginolyticus after 1 day, respectively. It is therefore concluded that L. vannamei that received chitin at 6 μg g−1 or chitosan at 4 μg g−1 or less increased its immune ability and resistance to V. alginolyticus infection.
關聯: 19(3), pp.191-204
URI: http://ntour.ntou.edu.tw/handle/987654321/26817
显示于类别:[水產養殖學系] 期刊論文

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