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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26812

Title: The peroxinectin of white shrimp Litopenaeus vannamei is synthesised in the semi-granular and granular cells, and its transcription is up-regulated with Vibrio alginolyticus infection
Authors: Chun-Hung Liu;Winton Cheng;Jiann-Chu Chen
Contributors: NTOU:Department of Aquaculture
Keywords: Peroxinectin;Litopenaeus vannamei;Semi-granular and granular cells;Vibrio alginolyticus;In situ hybridisation
Date: 2005-05
Issue Date: 2011-10-21T02:18:50Z
Publisher: Fish & Shellfish Immunology
Abstract: Abstract:Peroxinectin mRNA expression in the different types of haemocytes of white shrimp Litopenaeus vannamei was studied by in situ hybridisation using digoxigenin-UTP-labelled riboprobes and reverse transcription-polymerase chain reaction (RT-PCR). Granular cells (GC) and a mixture of semi-granular cells (SGC) and hyaline cells (HC) were separated by 70% Percoll gradient centrifugation. Peroxinectin was synthesised in both GC and the mixture of SGC-HC. An in situ hybridisation assay indicated that peroxinectin mRNA expression occurred in GC and SGC, but not in HC. Peroxinectin transcript up-regulated significantly, whereas haemocyte count decreased significantly at 6, 12 and 24 h post Vibrio alginolyticus-injection with slower restoration as compared to that of saline-injected shrimp. The RT-PCR assay indicated that peroxinectin exists extensively in several species of decapod crustaceans including L. vannamei, freshwater prawn Macrobrachium rosenbergii, common caridina Caridina pseudodenticulata, stone crab Thalamita crenata and mud crab Scylla serrata suggesting that this protein plays an important role in defence against pathogens.
Relation: 18(5), pp.431-444
URI: http://ntour.ntou.edu.tw/handle/987654321/26812
Appears in Collections:[水產養殖學系] 期刊論文

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