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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26796

Title: Suppression of myostatin with vector-based RNA interference causes a double-muscle effect in transgenic zebrafish
Authors: Chiou-Yueh Lee;Shao-Yang Hu;Hong-Yi Gong;Mark Hung-Chih Chen;Jenn-Kan Lu;Jen-Leih Wu
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: Myostatin;Zebrafish;RNA interference
Date: 2009-10-02
Issue Date: 2011-10-21T02:18:43Z
Publisher: Biochemical and Biophysical Research Communications
Abstract: Abstract:Myostatin belongs to the transforming growth factor (TGF)-β superfamily and is a potent negative regulator of skeletal muscle development and growth. We utilized microinjection of an antisense RNA-expressing vector to establish a hereditarily stable myostatin gene knockdown zebrafish strain with a double-muscle phenotype. Real-time PCR and immunostaining revealed that the myostatin messenger (m)RNA and protein levels in homozygous transgenic zebrafish were 33% and 26% those of the non-transgenic controls, respectively. Also, the mRNA levels of myogenic regulatory factor markers such as MyoD, myogenin, Mrf4, and Myf5 were dramatically elevated in myostatin-suppressed transgenic fish compared to the non-transgenic controls. Although there was no significant difference in body length, homozygous transgenic zebrafish were 45% heavier than non-transgenic controls. Histochemical analysis showed that the cross-sectional area of the muscle fiber of homozygous transgenic fish was twice as large as that of non-transgenic controls. This is the first model zebrafish with a hereditarily stable myostatin-suppressed genotype and a double-muscle phenotype.
Relation: 387(4), pp.766-771
URI: http://ntour.ntou.edu.tw/handle/987654321/26796
Appears in Collections:[水產養殖學系] 期刊論文

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