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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26781

Title: Response to acute changes in salinity of two different muscle type creatine kinase isoforms, from euryhaline teleost (Oreochromis mossambicus) gills
Authors: Hong-Yi Gong;Jen-Leih Wu;Wei-Tung Huang;Cliff Ji-Fan Lin;Ching-Feng Weng
Contributors: NTOU:Department of Aquaculture
Keywords: Creatine kinase;Muscle type CK isoform;Salinity;Gill;Mozambique tilapia (Oreochromis mossambicus)
Date: 2004-11-18
Issue Date: 2011-10-21T02:18:38Z
Publisher: Biochimica et Biophysica Acta (BBA) - General Subjects
Abstract: Abstract:Two CKM isoforms (CKM1 and CKM2) from the gills of tilapia (Oreochromis mossambicus) were obtained after transfer from freshwater (FW) to seawater (SW, 25 ppt). Based on the 5′ and 3′ RACE, the identity of CKM1 and CKM2 was determined to be 59% in the 5′-untranslated region (5′-UTR) and 41.9% in the 3′-UTR. Using Northern blot hybridization with the CKM1 and CKM2 3′-UTR probes, CKM1 and CKM2 were found to be expressed in muscle, heart and gill. The levels of these two different CK isoforms (CKM1 and CKM2) were shown to be different in FW than after acute SW transfer, showing that CKM isoforms respond to changes in salinity.
Relation: 1675(1-3), pp.184-191
URI: http://ntour.ntou.edu.tw/handle/987654321/26781
Appears in Collections:[水產養殖學系] 期刊論文

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