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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26773

Title: Steroid hormones (17β-estradiol and hydrocortisone) upregulate hepatocyte nuclear factor (HNF)-3β and insulin-like growth factors I and II expression in the gonads of tilapia (Oreochromis mossambicus) in vitro
Authors: Wei-Tung Huang;Hao-Cheng Yu;Chih-Chien Hsu;Ching-Fong Liao;Hong-Yi Gong;Cliff Ji-Fan Lin;Jen-Leih Wu;Ching-Feng Weng
Contributors: NTOU:Department of Aquaculture
Keywords: Hepatocyte nuclear factor (HNF);Gonads;Steroids;Immunohistochemistry
Date: 2007-10-15
Issue Date: 2011-10-21T02:18:36Z
Publisher: Theriogenology
Abstract: Abstract:Hepatocyte nuclear factors (HNF-1α, -1β and -3β) and insulin-like growth factors (IGF-I and -II), which are involved in liver-specific gene expression, metabolism, development and cell growth, have been found in the gonads of tilapia (Oreochromis mossambicus). However, the functions of these factors and how they interact within the gonads of bony fish are not understood. In the present study, we provided experimental evidence that the expression of HNF-3β in the gonads of tilapia, but not HNF-1α and -1β, was affected in vitro by 17β-estradiol and hydrocortisone. Immunohistochemical staining confirmed that tilapia HNF-3β was mainly found in the nuclei of hepatocytes, the follicular granulosa cells of the ovaries, and the interstitial cells of the testes of adult tilapia. Further data were gathered at various steroid concentrations (0.1, 1, 10, 100, and 1000 nM) over various culture intervals (6, 12, 18, 24, 30, and 36 h) and subjected to semi-quantitative RT-PCR analysis. The expression of downstream genes (IGF-I and -II) followed the same temporal patterns as HNF-3β, albeit at decreased levels for 30 and 36 h culture intervals. Both hormones upregulated HNF-3β mRNA expression at concentrations of 0.1–10 nM, and reached optimal physiological concentrations for induction of IGFs at 1–10 nM. The identity of the PCR fragments was concurrently verified by sequencing and PCR-Southern hybridization. We inferred that HNF-3β and IGFs may play a regulatory role in tilapia gonads during oocyte maturation and spermatogenesis.
Relation: 68(7), pp.988-1002
URI: http://ntour.ntou.edu.tw/handle/987654321/26773
Appears in Collections:[水產養殖學系] 期刊論文

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