National Taiwan Ocean University Institutional Repository:Item 987654321/26766
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题名: Reverse transcription loop-mediated isothermal amplification for rapid and sensitive detection of nervous necrosis virus in groupers
作者: Chia-Hsuan Sung;Jenn-Kan Lu
贡献者: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
关键词: Nervous necrosis virus;NNV;Reverse transcription loop-mediated isothermal amplification;RT-LAMP;Epinephelus coioides;Epinephelus lanceolatus
日期: 2009-08
上传时间: 2011-10-21T02:18:34Z
出版者: Journal of Virological Methods
摘要: Abstract:The reverse transcription loop-mediated isothermal amplification (RT-LAMP) method is a sensitive nucleic acid diagnostic method that can amplify rapidly a target template; it can be applied for the diagnosis of viral disease in grouper aquaculture. In this study, two outer and two inner primers were designed from nervous necrosis virus (NNV) coat protein gene sequence. The reaction temperature and time for the detection of NNV were optimized at 65 °C for 60 min. The detection limit of RT-LAMP is 10−6 NNV-RNA from infected groupers, and more sensitive than the one-step RT-PCR and nested RT-PCR. The combination of RNA rapid extraction and RT-LAMP, the process can be completed within 2 h. Thus, the RT-LMAP is a rapid, sensitive, specific and efficient method for detection of NNV in groupers.
關聯: 159(2), pp.206-210
URI: http://ntour.ntou.edu.tw/handle/987654321/26766
显示于类别:[水產養殖學系] 期刊論文

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