National Taiwan Ocean University Institutional Repository:Item 987654321/26741
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题名: Identification of six common species of processed filefish using cytochrome b gene sequence and PCR-RFLP analysis
作者: Wu Y. J.;C. H. Hsieh;H. M. Chen;D. F. Hwang
贡献者: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
关键词: File fish;Cytochrome b gene;PCR-RFLP;Species identification
日期: 2008
上传时间: 2011-10-21T02:18:29Z
出版者: The Raffles bulletin of zoology
摘要: Abstract:Sequence analysis and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) technique were used to identify the commercial species of Aluterus monoceros, Aluterus scriptus, Monacanthus chinensis, Thamnaconus hypargyreus, Thamnaconus modestus and Chaetodermis penicilligerus. The primers L14735 and H15149ad were designed in the mitochondrial cytochrome b gene and the molecular weight of amplified fragment from processed file fish meats was 465 bp. Furthermore, the results obtained from the HaeIII enzyme digestion could be used to distinguish the six commercial file fish species in fresh and heat-treated meats. Using the PCR-RFLP method, six file fish species could be successfully identified in fresh and heat-treated meats. The PCR-RFLP technique developed in this study was proved to be a rapid, reliable and simple method that enables easy and accurate identification of six commercial file fish processed species.
關聯: 19, pp.151-158
URI: http://ntour.ntou.edu.tw/handle/987654321/26741
显示于类别:[水產養殖學系] 期刊論文

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