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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26737

Title: Identification and phylogenetic analysis on lipopolysaccharide and β-1,3-glucan binding protein (LGBP) of kuruma shrimp Marsupenaeus japonicus
Authors: Yong-Chin Lin;Baskaralingam Vaseeharan;Jiann-Chu Chen
Contributors: NTOU:Department of Aquaculture
Keywords: Lipopolysaccharide (LPS) and β-1, 3-glucan binding protein (LGBP);Marsupenaeus japonicus;LPS injection
Date: 2008
Issue Date: 2011-10-21T02:18:28Z
Publisher: Developmental and Comparative Immunology
Abstract: Abstract:A lipopolysaccharide (LPS) and β-1,3-glucan binding protein (LGBP) gene was cloned from hemocytes of kuruma shrimp Marsupenaeus japonicus by reverse-transcription polymerase chain reaction (RT-PCR), cloning and sequencing of overlapping PCR, and rapid amplification of cDNA ends (RACE) method. The open reading frame (ORF) of M. japonicus LGBP is 1062 bp and encodes a 354 amino acid (aa) sequence with a 23 aa signal peptide. The calculated molecular mass of the mature protein (331 aa) is 40.15 kDa with an estimated pI of 4.78. The M. japonicus LGBP sequence contains (1) two putative N-linked glycosylation sites, (2) two putative integrin-binding motifs, (3) a kinase C phosphorylation site (KCPS), (4) a glucanase motif (GM), and (5) two potential polysaccharide recognition motifs (polysaccharide binding motif (PsBM) and β-glucan recognition motif (GRM)), and with features of tryptophan-rich, slight homology to lysozyme, and slight homology to lectin. A sequence comparison showed that the deduced amino acids of M. japonicus LGBP has an overall high similarity to penaeid LGBP and βGBP (85.6–89.9%), lobster Homarus gammarus βGBP (77.0%), and crayfish Pacifastacius leniusculus LGBP (67.8%). The phylogenetic analysis revealed that M. japonicus LGBP grouped together with other crustacean LGBP and βGBP, and was close to termite GNBP, but was far way from moth βGBP, βGRP, fly GNBP, and mosquito βGRP. The LGBP of M. japonicus was strongly expressed in hemocytes. The LGBP mRNA transcript in hemocytes of M. japonicus was significantly upregulated 12–48 h after a LPS injection, indicating activation of the innate immune system through the binding of the LGBP and LPS complex.
Relation: 32(11), pp.1260-1269
URI: http://ntour.ntou.edu.tw/handle/987654321/26737
Appears in Collections:[水產養殖學系] 期刊論文

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