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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26736

Title: IPNV VP5, a Novel Anti-apoptosis Gene of the Bcl-2 Family, Regulates Mcl-1 and Viral Protein Expression
Authors: Jiann-Ruey Hong;Hong-Yi Gong;Jen-Leih Wu
Contributors: NTOU:Department of Aquaculture
Keywords: infectious pancreatic necrosis virus;apoptosis;Bcl-2;Mcl-2;VP5;virus replication
Date: 2002-04-10
Issue Date: 2011-10-21T02:18:28Z
Publisher: Virology
Abstract: Abstract:VP5, a 5′-terminal, small open reading frame in segment A of the aquatic birnavirus (infectious pancreatic necrosis virus, IPNV) genome, encodes a 17-kDa nonstructural protein. We previously reported apoptosis induced by IPNV in a fish cell line. In the present study, we cloned and identified VP5 and tested its function. Comparisons of the amino acid sequence of VP5 with well-known Bcl-2 family member proteins showed that the VP5 protein contains Bcl-2 homology (BH) domains BH1, BH2, BH3, and BH4 but without the transmembrane region. VP5-stable clones enhanced viability, prevented membrane blebbing, delayed DNA internucleosomal cleavage, and decreased virus titer during IPNV infection but, when deleted, BH domains 1 and 2 could lose the preventable ability. In addition, VP5 was demonstrated to be able to enhance or assist in maintaining the functional half-life of survival factor Mcl-1 and regulate specific viral protein expression during the early replication cycle. Finally, we found that VP5 was capable of enhancing cell viability when cells were exposed to UV irradiation. In summary, these results suggest that the aquatic birnavirus may utilize a notable strategy via VP5 to regulate the host apoptosis-off system for enhancing progeny production.
Relation: 295(2), pp.217-229
URI: http://ntour.ntou.edu.tw/handle/987654321/26736
Appears in Collections:[水產養殖學系] 期刊論文

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