National Taiwan Ocean University Institutional Repository:Item 987654321/26733
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題名: Inhibition of nervous necrosis virus propagation by fish Mx proteins
作者: Chih-Hung Lin;Joseph Abraham Christopher John;Cheng-Hui Lin;Chi-Yao Chang
貢獻者: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
關鍵詞: DNA vaccine;Nodavirus;Grouper;Mx
日期: 2006-12-15
上傳時間: 2011-10-21T02:18:27Z
出版者: Biochemical and Biophysical Research Communications
摘要: Abstract:Mx proteins are interferon induced, antiviral proteins, expressed in response to treatment with double stranded RNA or virus infection. Here we report the cloning, sequencing, and antiviral property of three forms of Mx genes, MxI, MxII, and MxIII from grouper (Epinephelus coioides). Multiple comparison of grouper Mx amino acid sequences revealed the conservation of Mx putative GTP-binding domain, dynamin family signature, and leucine zipper motif. We have established a new cell line, grouper brain 3 (GB3), and prepared stable clones expressing Flag-epitope tagged grouper MxI, MxII, and MxIII. Immunostaining shows that all the three grouper Mx proteins are localized in the cytoplasm. To examine the antiviral activity of grouper Mx proteins, these stable clones were infected by a nodavirus, yellow grouper nervous necrosis virus (YGNNV), and the results showed that all the three Mx isoforms have the efficiency of reducing the titre of virus from 10- to 100-fold. Moreover, through immunocytochemistry we demonstrated that Mx protein can inhibit the YGNNV propagation in GB3 cells. Taken together, this study demonstrates that grouper Mx proteins have efficient inhibitory activity against nodavirus, the most endangered virus of fish, and this information would be helpful to design effective DNA vaccines that can confer an early non-specific antiviral protection.
關聯: 351(2), pp.534-539
URI: http://ntour.ntou.edu.tw/handle/987654321/26733
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