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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/26687

Title: Cloning and Characterization of a Novel Nuclear Bcl-2 Family Protein, zfMcl-1a, in Zebrafish Embryo
Authors: Ming-Chyuan Chen;Hong-Yi Gong;Ching-Yi Cheng;Jia-Pey Wang;Jiann-Ruey Hong;Jen-Leih Wu
Contributors: NTOU:Department of Aquaculture
Keywords: apoptosis;Bcl-2 family proteins;nuclear Mcl-1;zebrafish;embryogenesis
Date: 2000-12-20
Issue Date: 2011-10-21T02:18:18Z
Publisher: Biochemical and Biophysical Research Communications
Abstract: Abstract:The importance of the Bcl-2 family proteins in normal vertebrate embryogenesis is being recognized; however, their regulatory mechanism is poorly understood. To elucidate the embryonic roles of Bcl-2 family proteins, we cloned and characterized the first zebrafish Bcl-2 family protein, zfMcl-1a. Zebrafish Mcl-1a shows the highest homology to rat Mcl-1 and contains several conserved BH domains of the Bcl-2 family proteins. It also contains a nuclear localization signal (NLS). Using EGFP reporter analysis, we verified the nuclear localization of zfMcl-1a. Deletion of the NLS resulted in distribution of the fusion protein in the cytoplasm. Northern blot analysis indicated that zfMcl-1a mRNA is 1.5 kb and was expressed in oocytes and throughout embryonic development. Notably, the expression of zfMcl-1a transcript was significantly downregulated during gastrulation. These results suggest that zfMcl-1a is a novel nuclear Bcl-2 family protein and is likely to play an important role in zebrafish oogenesis and embryogenesis.
Relation: 279(2), pp.725-731
URI: http://ntour.ntou.edu.tw/handle/987654321/26687
Appears in Collections:[水產養殖學系] 期刊論文

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