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|Title: ||Cloning and biological analysis of the zebrafish (Danio rerio) insulin-like growth factor binding protein-2 proximal promoter region|
|Authors: ||Jyh-Yih Chen;Ming-Jun Chou;Hong-Yi Gong;Tzou-Chi Huang;Jen-Leih Wu;Ching-Ming Kuo|
|Issue Date: ||2011-10-21T02:18:16Z
|Publisher: ||DNA and Cell Biology|
|Abstract: ||Abstract:Insulin-like growth factor binding protein 2 (IGFBP-2) plays an important role in the regulation of IGF's action and endocrinology in fish. To understand the molecular mechanism which controls transcription of the IGFBP-2 gene, we cloned and sequenced the IGFBP-2 proximal promoter region of the zebrafish IGFBP-2 gene and characterized its activity by firefly luciferase transient transfection expression assays. Different fragments of the zebrafish IGFBP-2 5'-flanking region were transfected into Hela and ZFL cells. In these cell lines, maximum promoter activity was located in the 900 base pairs (bp) of the zebrafish IGFBP-2 5' flanking region in the ZFL cell line and 318 bp of the zebrafish IGFBP-2 5' flanking region in the Hela cell line. The in vivo actions of the IGFBP-2 promoter on developmental stage expression were further investigated in transgenic zebrafish in which an IGFBP-2 (900-bp) promoter-driven green fluorescent protein encoding the GFP cDNA transgene was microinjected into zebrafish embryos. Morphological and RT-PCR studies of transgenic zebrafish indicated that the IGFBP-2 promoter-driven GFP transcripts appeared for the first time in the 32-cell stage. These results indicate that the IGFBP-2 promoter is active in a development-specific manner. These results suggest that the IGFBP-2 promoter plays an important role in teleost embryo growth.|
|Relation: ||24(3), pp.199-208|
|Appears in Collections:||[水產養殖學系] 期刊論文|
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