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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/17432

Title: 探討Thermoanaerobacterium sp. NTOU2 利用不同醣類醱酵生產乙醇之研究
Ethanol Production From Different Sugars by Thermoanaerobacterium sp. NTOU2
Authors: Wei-Chieh Chen
陳偉傑
Contributors: NTOU:Institute of Marine Biology
國立臺灣海洋大學:海洋生物研究所
Keywords: 醣類利用;酵母粉濃度;生產乙醇;酸水解物
Thermoanaerobacterium sp. NTOU2;Sugars Metabolized;Yeast extract;Ethanol production;Acid hydrolysates
Date: 2008
Issue Date: 2011-07-04
Abstract: 本論文以一株分離自龜山島海底熱泉中,厭氧嗜熱醣類分解菌Thermoanaerobacterium sp. NTOU2作為研究目標,其最適生長溫度為70℃,可利用五碳醣、六碳醣與多醣為生長碳源,乙醇為主要代謝產物之一。此菌株最適培養條件為培養基中緩衝溶液以10 mM KH2PO4/K2HPO4組合9 g/L NaHCO3-CO2。培養基中添加0.5 % 澱粉或0.5 % 葡萄糖組合 0.5 % 木糖,其生長表現及乙醇產量較使用其他醣類濃度為佳。培養基pH值則以0.5 % 葡萄糖或0.5 % 木糖於pH 6.5或pH 6.8培養條件下,其生長表現及乙醇生產量最佳。在醣類濃度與酵母粉濃度方面,以0.5 % 葡萄糖或0.5 % 木糖分別組合0.5 % 酵母粉,1 % 澱粉組合1 % 酵母粉,其生長表現及乙醇生產量最佳。木質纖維素酸水解時的副產物甲酸、醋酸、呋喃甲醛、5-羥甲基糠醛、果糖酸對此菌株利用醣類生產乙醇方面,發現0.5 % 甲酸、0.3 % 醋酸、0.05 % 呋喃甲醛、0.75 % 5-羥甲基糠醛及0.5 % 果糖酸存在時都會影響此菌利用0.5 % 葡萄糖時生產乙醇的產量,而0.5 % 甲酸、0.4 % 醋酸、0.1 % 呋喃甲醛、0.5 % 5-羥甲基糠醛及0.5 % 果糖酸則會影響此菌利用0.5 % 木糖時生產乙醇的產量。
In this report a new thermophilic anaerobic bacterium Thermoanaerobacterium sp. NTOU2 from Gueishan island which was capable of growth utilizing hexose and pentose at 70 ℃ was used to investigate its potential for ethanol production/yield from sugars. When using 10 mM KH2PO4/K2HPO4+9 g/L NaHCO3–CO2 as buffer in medium, the growth of strain NTOU2 were faster than other buffer. When the strain NTOU2 using 0.5 % starch or 0.5 % glucose + 0.5 % cellobiose as the substrate, the ethanol production were highest than other sugar. When using 0.5 % glucose and 0.5 % xylose as the substrate, the highest ethanol production by strain NTOU2 is at pH 6.5. The highest ethanol production was found in the medium containing 0.5 % glucose + 0.5 % yeast extract, 0.5 % xylose + 0.5 % yeast, 1.0 % starch and 1.0 % yeast. The presence of 0.75 % or higher concentration of 5-HMF, 0.5 % or higher concentration of levulinic acid, 0.05 % or higher concentration of furfural, 0.5 % or higher concentration of formic acid, and 0.3 % or higher concentration of acetic acid sharply inhibited the growth and ethanol production by strain NTOU2 when 0.5 % glucose was used as the substrate. The presence of 0.5 % or higher concentration of 5-HMF, 0.5 % or higher concentration of levulinic acid, 0.1 % or higher concentration of furfural, 0.5 % or higher concentration of formic acid, and 0.4 % or higher concentration of acetic acid sharply inhibited the growth and ethanol production by strain NTOU2 when 0.5 % xylose was used as the substrate.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M94340004
http://ntour.ntou.edu.tw/ir/handle/987654321/17432
Appears in Collections:[海洋生物研究所] 博碩士論文

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