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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/16939

Title: NK1接受器對麻醉鼠重複刺激所引起骨盆神經-尿道反射增益現象之影響
Effects of NK1 receptor on Repetitive Stimulation-Induced Pelvic-Urethra Reflex Potentiation in Anesthetized Rats
Authors: Hui-Fang Chiu
邱惠芳
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: 物質P;骨盆尿道反射;脊髓反射增益現象
Substance P;PUR;SRP;NK 1 receptors
Date: 2008
Issue Date: 2011-06-30T08:45:08Z
Abstract: Neurokinin 1 (NK1)接受器在脊髓背角的調控表現被認為和脊髓的痛覺調控有關。我們測試NK1接受器否經由PLC/Ca2+去調控脊髓反射增益現象 (spinal reflex potentiation; SRP)。首先先給予1/30赫茲作為單一刺激誘發出一個骨盆神經-尿道反射 (Pelvic-urethral Reflex; PUR)的反射現象,接著改成1赫茲的重複性刺激以誘發出增益現象。脊髓腔內注射Substance P (0.1 μM, 10 μl),為NK1接受器的作用劑,在單一刺激下發現產生SRP,接著脊髓腔內注射PLC抑制劑Neomycin (100 μM, 10 μl) ,發現SRP有被抑制住的情形,接著脊髓腔內注射細胞內的鈣離子釋放劑Caffeine (100 μM, 10 μl),發現SRP又再度被活化。接著脊髓腔內注射NK1接受器的作用劑Hemokinin 1 (0.1 μM, 10 μl),在單一刺激下發現產生SRP,接著脊髓腔內注射Neomycin (100 μM, 10 μl),發現被Hemokinin 1所誘導產生的SRP有被抑制住的情形,接著脊髓腔內注射Caffeine (100 μM, 10 μl),發現被Neomycin抑制的SRP又再度被活化。脊髓腔內注射NK1接受器的拮抗劑WIN51708 (100μM, 10μl),發現在重複刺激誘發所產生的SRP有被抑制的情形,接著脊髓腔內注射PLC活化劑m-3M3FBS (100 μM, 10 μl),發現被WIN51708抑制的SRP有再度活化的情形,接著脊髓腔內注射細胞內的鈣離子阻斷劑Dantrolene (100 μM, 10 μl),發現被m-3M3FBS 所活化的SRP又再度被抑制。這些研究顯示出,NK1接受器參與痛覺過敏與疼痛異常的情形可能是藉由活化PLC路徑所引發的Ca2+釋放,而造成SRP產生所調控。
Neurokinin 1 (NK1) receptors are expressed in dorsal horn neurons is recognized to mediate the transmission and modulation of nociception at the spinal cord level. However, the possibility that the NK1 receptor and the downstream intracellular cascade may modulate the induction of the spinal reflex potentiation (SRP), a novel form of activity-dependent neural plasticity mediating post-inflammatory hypergesia and tactile allodynia, has been yet to be established. We test the hypothesis that NK1 and the downstream intracellular PLC/Ca2+ cascade may modulate the induction of SRP using pharmacological approach in in vivo animal preparations. When compared with test stimulation (1/30 Hz) evoked a baseline reflex activity with a single action potential, repetitive stimulation (1 Hz) induced SRP in the reflex activity. Intrathecal Substance P, a Neurokinin 1 receptor agonists (0.1μM, 10μl), induced excitatory effects on the test stimulation (TS, 1/30 Hz) elicited reflex activities, exhibited inhibitory effects reversed by intrathecal Neomycin, a phospholipase C inhibitor (100μM, 10μl). The reversal effect on SRP caused by the Neomycin was reactivation by intrathecal Caffeine, an intracellular calcium release (100μM, 10μl). Intrathecal Hemokinin 1, a Neurokinin 1 receptor agonists (0.1μM, 10μl), induced excitatory effects on the test stimulation (TS, 1/30 Hz) elicited reflex activities, exhibited inhibitory effects reversed by intrathecal Neomycin, a phospholipase C inhibitor (100μM, 10μl). The reversal effect on SRP caused by the Neomycin was reactivation by intrathecal Caffeine, an intracellular calcium release (100μM, 10μl). Intrathecal WIN51708, a NK1 receptor antagonist (100μM, 10μl), exhibited inhibitory effects on the RS-induced SRP that was reversed by intrathecal m-3M3FBS, a phospholipase C activator (100μM, 10μl). The reversal effect on SRP caused by the m-3M3FBS was abolished by intrathecal Dantrolene, an intracellular calcium blocker (100μM, 10μl). These findings suggested that through the Ca2+ release triggered by the phospholipase C activation activating, NK1 receptor may modulate the repetitive stimulation-induced SRP, which is presumed to be involved in post-inflammatory hypergesia and tactile allodynia.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M95330052
http://ntour.ntou.edu.tw/ir/handle/987654321/16939
Appears in Collections:[水產養殖學系] 博碩士論文

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