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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/16868

Title: 點帶石斑在鹽度與溫度緊迫下其免疫反應及對於弧菌Vibrio alginolyticus 之感受性
The immune responses of orange-spotted grouper Epinephelus coioides and its susceptibility to Vibrio alginolyticus under thermal and salinity stresses
Authors: Shao-An Cheng
鄭紹安
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: 鹽度;溫度;環境;免疫;點帶石斑
salinity;temperature;environment stress;immunity;Orange-spotted grouper;Epinephelus coioides
Date: 2008
Issue Date: 2011-06-30T08:43:34Z
Abstract: 本論文的目的在於研究點帶石斑Epinephelus coioides經鹽度以及溫度緊迫後的免疫因子反應,以及對於Vibrio alginolyticus之感受性。 點帶石斑由鹽度34‰的海水分別移入鹽度34‰ (對照組)、20以及6‰的水中,經過3、6、12、24、48、96小時,分析其白血球數、呼吸爆活性、吞噬能力、替代路徑補體活性(ACH50)和溶菌酵素活性,發現石斑魚移入鹽度20‰的環境,其白血球數、呼吸爆活性和吞噬率的細胞性免疫反應因子均有提升,但移入 6‰的環境則皆下降。石斑魚由34‰移入6‰及20‰水中,替代路徑補體活性和溶菌酵素活性的體液性免疫因子均顯著下降。另外,石斑魚經感染V. alginolyticus (每隻魚感染劑量為2.3×109 cfu),分別由34‰移入34‰ (對照組)、20‰、6‰水中,經5天的vibrio攻擊實驗,觀察石斑魚的活存狀況,發現石斑魚由34‰移入34‰及20‰水中,其活存率沒有顯著差異,但由34‰移入6‰的水中的石斑魚,其活存率顯著低於對照組 (34‰)。證實石斑魚在受到低鹽度緊迫下,其免疫力下降,抵抗V. alginolyticus感染能力減弱。 點帶石斑由水溫27 ℃的環境,移至水溫19 ℃、27 ℃(對照組)和35 ℃的環境,經過12、24、48和96小時,分析其呼吸爆活性、吞噬能力、替代路徑補體活性和溶菌酵素活性。發現石斑魚移入低溫(19 ℃) 和高溫(35 ℃)的環境,其白血球數、呼吸爆活性和吞噬率的細胞性免疫反應因子、替代路徑補體活性和溶菌酵素活性的體液性免疫反應因子皆下降。另外,石斑魚經感染V. alginolyticus (每隻魚感染劑量為2.3×109 cfu),分別由27 ℃(對照組)移入19 ℃、27 ℃、35 ℃的海水中,經5天,觀察其活存狀況,發現石斑魚由27 ℃移入19 ℃和35 ℃的海水中,其活存率顯著低於由27 ℃移入 27 ℃(對照組)的水中。証實石斑魚由27 ℃移入低溫(19 ℃)或高溫(35 ℃)的緊迫下免疫力下降,抵抗V. alginolyticus的能力下降。 本實驗證實,石斑魚受到環境緊迫其白血球數減少,細胞性免疫因子與體液性免疫反應因子皆下降,抵抗細菌的能力下降。建議避免石斑魚養殖在過高或過低溫突變的環境,而養殖的鹽度可在20-34‰,避免由34‰移到20‰或以下,甚至鹽度6‰,在此低鹽度環境下緊迫,石斑魚的免疫影響甚大。本研究結果有助於石斑魚的養殖環境的管理,減少養殖環境緊迫造成石斑魚免疫力下降及病原感染而爆發疾病,得以增加養殖生產效益。
Orange-spotted grouper Epinephelus coioides held in 34‰ seawater were then cultured onward at 6‰, 20‰ and 34‰ (control), and were examined for non-specific cellular and humoral responses after 3, 6, 12, 24, 48 and 96 h. Total leucocyte count increased significantly when fish were transferred to 20‰ after 3~48 h, but decreased significantly when fish were transferred to 6‰ after 3~96 h. Concerning the non-specific cellular response, respiratory burst increased significantly when fish were transferred to 20‰ over 3~96 h, phgaocytic rate increased significantly when fish were transferred to 20‰ after 3~24 h. However, respiratory burst and phagocytic rate decreased significantly when fish were transferred to 6‰ after 3~96 h. Concerning the non-specific humoral response, lysozyme activity and alternative complement pathway (ACH50) decreased significantly when fish were transferred to 20‰ and 6‰ after 3~96 h. E. coioides that received Vibrio alginolyticus at 2.3 × 109 colony-forming units (cfu) fish-1 and then placed in seawater of 34‰, 20‰ and 6‰ was examined for mortality. Survived of fish that transferred to 6‰ seawater was significantly lower than that of fish that transferred to 34‰ and 20‰ over 48~120 h. It was concluded that E. coicoides subjected to salinity change from 34‰ to low salinity (6‰) decreased its immunity and its resistance against V. alginolyticus. E. coioides held in 27 ℃ seawater were then cultured onward at 19 ℃, 27 ℃ (control) and 35 ℃, and were examined for non-specific cellular and humoral responses at 12, 24, 48 and 96 h. Total leucocyte count decreased significantly when fish were transferred to 19 ℃ after 48 to 96 h, and when fish were transferred to 35 ℃ after 24~96 h. Concerning the non-specific cellular response, respiratory burst decreased significantly when fish were transferred to 19 ℃ and 35 ℃ over 48~96 h, and phagocytic rate decreased significantly when fish were transferred to 19 ℃, and 35 ℃ over 24~96 h. Concerning the non-specific humoral response, lysozyme activity and alternative complement pathway (ACH50) decreased significantly when fish were transferred to 19 ℃ and 35 ℃ after 48~96 h. E. coioides that received V. alginolyticus at 2.3 × 109 cfu fish-1 and then placed in seawater of 19 ℃, 27 ℃ and 35 ℃ was examined for mortality. Survival of fish that transferred to 19 ℃ and 35 ℃ was significantly lower than that in the control temperature (27 ℃) after 24~120 h. It was concluded that E. coioides subjected to temperature change from 27 ℃ to low temperature (19 ℃) and high temperature (35 ℃) decreased its immunity and its resistance against V. alginolyticus.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M95330042
http://ntour.ntou.edu.tw/ir/handle/987654321/16868
Appears in Collections:[水產養殖學系] 博碩士論文

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