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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/16717

Title: 莫三比克吳郭魚(Oreochromis mossambicus)在低溫與高溫緊迫下之免疫反應及對於Streptococcus iniae之感受性
The immune response of Mozambique tilapia Oreochromis mossambicus and its susceptibility to Streptococcus iniae under stress in low and high temperatures
Authors: Diegane Ndong
龍成功
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: 莫三比克吳郭魚;免疫反應;低溫;高溫
Mozambique tilapia;Oreochromis mossambicus;Temperature;Challenge Non-specific cellular response;Non-specific humoral response;Streptococcus iniae
Date: 2005
Issue Date: 2011-06-30T08:41:29Z
Abstract: 中文摘要 蓄養於27 ℃莫三比克吳郭魚Oreochromis mossambicus分別移入19、23、27(對照組)及35 ℃水中,經過12-96 h,檢測非特異性細胞免疫反應及體液性免疫反應,結果發現移入19和23 ℃水中,分別經過48和96 h,以及移入35 ℃水中經過12-96 h,魚之白血球顯著下降。 關於非特異性細胞免疫反應方面,魚由27 ℃移入19、31和35 ℃,經過24-96 h,其呼吸爆顯著下降;但移到低溫(19和23 ℃)及高溫(31和35 ℃)水中,經12-96 h,吞噬活性及吞噬指標顯著下降。 關於非特異性體液免疫反應方面,吳郭魚由27 ℃移到19 ℃,經過12、24、48及96 h,lysozyme活性顯著下降;但移到31及35℃水中的魚,經過48及96 h,lysozyme活性顯著增加。移到19和23 ℃,經過12 h,魚之補體活性(ACH50)顯著下降。但移到31及35℃,經過24 h,魚之補體活性顯著增加。統計結果顯示移到19和35 ℃水中的魚,吞噬指標及呼吸爆明顯與對照組(27 ℃)不一樣,也顯示魚由27 ℃移到19和35 ℃水中時,免疫活性下降。 另外執行一個實驗,蓄養於27 ℃水中莫三比克吳郭魚,注射培養於TSB的細菌Streptococcus iniae,使魚的感染劑量為1x107 cfu fish-1後,分別將魚移到19、23、27、31和35 ℃水中,經過48-168 h,發現經細菌處理的吳郭魚在移到19和35 ℃水中,死亡率明顯高於移入23、27和31℃水中的魚。 本研究證實飼養於27 ℃水中莫三比克吳郭魚O. mossambicus,在經低溫變化(19、23 ℃)和高溫變化(31、35 ℃),其免疫力下降,本實驗也證實飼養於27 ℃水中的莫三比克吳郭魚,在水溫約8 ℃的變化,如移到19和35 ℃時,對細菌感染之抵抗力顯著下降。
Abstract Mozambique tilapia Oreochromis mossambicus held in 27 ºC were then cultured onward at 19, 23, 27 (control), 31 and 35 ºC, and were examined for non-specific cellular and humoral responses at 12-96 h. Total leucocyte count decreased significantly when fish were transferred to 19 and 23 ºC after 48 and 96 h, and when transferred to 35 ºC over 12-96 h, respectively. Concerning the non-specific cellular response, respiratory burst decreased significantly when fish were transferred to 19, 31 and 35 ºC over 24-96 h, whereas phgaocytic activity and phagocytic index decreased significantly when fish were transferred to low temperatures (19 and 23 ºC) and high temperatures (31 and 35 ºC) over 12-96 h. Concerning the non-specific humoral response, lysozyme activity decreased significantly when fish were transferred to 19 ºC after 12, 24, 48 and 96 h, but increased significantly when fish were transferred to 31 and 35 ºC over 48-96 h. Alternative complement pathway (ACH50) decreased significantly when fish were transferred to 19 and 23 C after 12 h, but increased when transferred to 31 and 35 ºC after 24 h. The multivariate discriminant analysis revealed that decreases in phagocytic index and respiratory burst were responsible for separating the two temperature groups (19 and 35 oC) from the control group (27 oC) indicating that tilapia when transferred from 27 oC to 19 and 35 oC reduce their immunity. In another experiment, tilapia held at 27 ºC were injected with Streptococcus iniae grown in tryptic soy broth (TSB) at a dose of 1x107 colony-forming units (CFU) fish-1, and then reared onward at water temperatures of 19, 23, 27 (control), 31 and 35 ºC. Over 48-168 h, the cumulative mortality of S. iniae injected–fish held in 19 and 35 ºC was significantly higher than that of injected-fish held in 23, 27 and 31 ºC. It was concluded that transfer of tilapia O. mossambicus from 27 ºC to low temperatures (19 and 23 ºC) after 12 h, and when transferred to high temperatures (31 and 35 ºC) reduced their immunity. Moreover, tilapia under temperature stress at 19 and 35 ºC from 27 ºC decreased its resistance against S. iniae infection. Keywords: Mozambique tilapia; Oreochromis mossambicus; Temperature; Challenge Non-specific cellular response; Non-specific humoral response, Streptococcus iniae, Multivariate analysis
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M93330054
http://ntour.ntou.edu.tw/ir/handle/987654321/16717
Appears in Collections:[水產養殖學系] 博碩士論文

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