English  |  正體中文  |  简体中文  |  Items with full text/Total items : 28611/40652
Visitors : 753977      Online Users : 49
RC Version 4.0 © Powered By DSPACE, MIT. Enhanced by NTU Library IR team.
Scope Adv. Search
LoginUploadHelpAboutAdminister

Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/16627

Title: 重緣葉馬尾藻與小海帶熱水萃取物強化白蝦免疫力及抵抗Vibrio alginolyticus感染之研究
Administrations of hot-water extracts of Sargassum duplicatum and Petalonia binghamiae enhance the immune ability of white shrimp Litopenaeus vannamei and its resistance against Vibrio alginolyticus
Authors: Chiu-Hsia Li
李秋霞
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: 白蝦;重緣葉馬尾藻;小海帶;熱水萃取物;弧菌;免疫因子;感染實驗;吞噬作用;清除能力
Litopenaeus vannamei;Sargassum duplicatum;Petalonia binghamiae;Hot-water extract;Vibrio alginolyticus;Immune parameters;Challenge;Phagocytic activity;Clearance efficiency
Date: 2004
Issue Date: 2011-06-30T08:40:36Z
Abstract: 摘 要 白蝦Litopenaeus vannamei經重緣葉馬尾藻Sargassum duplicatum和小海帶Petalonia binghamiae之熱水萃取物注射或浸泡重緣葉馬尾藻熱水萃取物處理後,探討對白蝦非特異性免疫因子之影響以及抵抗Vibrio alginolyticus感染的效果。檢測免疫因子包含總血球數(total haemocyte count)、酚氧化酵素(phenoloxidase activity)、超氧離子(superoxide anion)、超氧歧化酵素活性(superoxide dismutase activity)、吞噬作用(phagocytic activity)及清除作用(clearance efficiency)。 以2、6、10及20 μg g-1劑量之重緣葉馬尾藻熱水萃取物注射白蝦,經一天後,發現其總血球數、酚氧化酵素活性及超氧離子均顯著增加。注射重緣葉馬尾藻熱水萃取物之白蝦,感染V. alginolyticus 1.8×106 cfu shrimp-1,經一天後,其吞噬作用及清除能力均亦明顯增加。白蝦注射重緣葉馬尾藻熱水萃取物後,一天後,注射V. alginolyticus 7×107 cfu ml-1,使其每隻白蝦感染濃度為1.4×106 cfu,並放置於34 □ 之海水中,發現經12小時後,經有重緣葉馬尾藻熱水萃取物之處理組,其存活率明顯高於注射生理食鹽水組及對照組,證實白蝦經重緣葉馬尾藻熱水萃取物處理後能增加其免疫力及抵抗V. alginolyticus之感染。 白蝦經浸泡100、300和500 mg-1重緣葉馬尾藻熱水萃取物,一小時後,發現經浸泡500 mg-1重緣葉馬尾藻熱水萃取物之白蝦,其總血球數、酚氧化酵素活性及超氧離子均顯著增加。白蝦浸泡重緣葉馬尾藻熱水萃取後,感染V. alginolyticus 1×106 cfu shrimp-1,並放置於34 □ 之海水中,48小時後,發現重緣葉馬尾藻熱水萃取物之處理組,其活存率明顯高於對照組。顯示白蝦經浸泡重緣葉馬尾藻熱水萃取物後,能增加其非特異性免疫力及其抵抗V. alginolyticus之感染。 白蝦經注射2、6、10及20 μg g-1小海帶熱水萃取物,經一天後,發現其總血球數、酚氧化酵素活性及超氧離子均有上升趨勢。注射小海帶熱水萃取物之白蝦,感染V. alginolyticus 1.8×106 cfu shrimp-1,經一天後,其吞噬作用及清除能力明顯增加。白蝦注射小海帶熱水萃取後,感染V. alginolyticus 1.4×106 cfu shrimp-1,並放置於34 □ 之海水中,48小時後,發現經有小海帶熱水萃取物之處理組,其活存率明顯高於生理食鹽水組及對照組。顯示白蝦經小海帶熱水萃取物處理後,能增加其非特異性免疫力及抵抗V. alginolyticus之感染。 本研究證實白蝦經注射2 μg g-1重緣葉馬尾藻或小海帶熱水萃取物處理後,以及經浸泡100 mg-1之重緣葉馬尾藻熱水萃取物處理一小時後,即具有增加白蝦免疫力,且增強其抵抗V. alginolyticus之感染。
This study monitored the immune response of white shrimp Litopenaeus vannamei ( 10.42 ± 1.39 g ) and its resistance against Vibrio alginolyticus when the shrimp were injected with hot-water extract of Sargassum duplicatum or Petalonia binghamiae at a dose of 2、6、10 or 20 μg g-1. The immune parameters included total haemocyte count (THC), phenoloxidase activity, respiratory burst (release of superoxide anion), superoxide dismutase activity, phagocytic activity and clearance efficiency to V. alginolyticus. L. vannamei injected with the hot-water extract of S. duplicatum at 2、6、10 and 20 μg g-1 showed increases in THC、phenoloxidase activity, respiratory burst and phagocytic activity and clearance efficiency to V. alginolyticus after 1 day. In another experiment, L. vannamei which had been injected with the hot-water extract of S. duplicatum, were challenged with V. alginolyticus at 1.4×106 colony-forming units (cfu) shrimp-1 and then placed in seawater of 34 □. The survival of shrimp that received the hot-water extract of S. duplicatum was significantly higher than that of shrimp injected with saline and the control shrimp at the termination of the experiment (6 days after the challenge). In the case that L. vannamei immersed with the hot-water extract of S. duplicatum at 100、300 and 500 mg l-1 showed increases in THC、phenoloxidase activity and respiratory burst after 1 day. In another experiment, L.vannamei which had been immersed with hot-water extract of S. duplicatum, were challenged with V. alginolyticus at 1×106 cfu shrimp-1 and then placed in seawater of 34 □. The survival of shrimp that immersed in hot-water extract of S. duplicatum was significantly higher than that of control shrimp at the termination of the experiment (6 days after the challenge). It is concluded that hot-water extract of S. duplicatum increased the immune ability of L. vannamei and resistance from V. alginolyticus infection. L. vannamei injected with hot-water extract of P. binghamiae showed increased THC、phenoloxidase activity and respiratory burst after 1 day. L. vannamei injected with hot-water extract of P. binghamiae showed increased phagocytic activity and clearance efficiency against V. alginolyticus after 1 days. In another experiment, L. vannamei which had been injected with hot-water extract of P. binghamiae were challenged with V. alginolyticus at 1.4×106 cfu shrimp-1, and then placed in seawater of 34 □. The survival of shrimp that received hot-water extract of P. binghamiae was significantly higher than that of shrimp injected with saline and control shrimp after 6 days. The present study indicated that both hot-water extracts of S. duplicatum and P. binghamiae can enhance the immune ability of L. vannamei and its resistance against V. alginolyticus infection. Further research is needed to evaluate the immunostimulatory effect of L. vannamei and its resistance against V. alginolyticus when shrimp feeding a diet containing either hot-water extract of S. duplicatum or P. binghamiae .
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M92330047
http://ntour.ntou.edu.tw/ir/handle/987654321/16627
Appears in Collections:[水產養殖學系] 博碩士論文

Files in This Item:

File Description SizeFormat
index.html0KbHTML204View/Open


All items in NTOUR are protected by copyright, with all rights reserved.

 


著作權政策宣告: 本網站之內容為國立臺灣海洋大學所收錄之機構典藏,無償提供學術研究與公眾教育等公益性使用,請合理使用本網站之內容,以尊重著作權人之權益。
網站維護: 海大圖資處 圖書系統組
DSpace Software Copyright © 2002-2004  MIT &  Hewlett-Packard  /   Enhanced by   NTU Library IR team Copyright ©   - Feedback