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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/16611

Title: 海水微藻抑制病原弧菌Vibrio alginolyticus增生之研究
Inhibitory effects of marine microalgae on the multiplication of Vibrio alginolyticus
Authors: Shu-Mei Lin
林舒美
Contributors: NTOU:Department of Aquaculture
國立臺灣海洋大學:水產養殖學系
Keywords: 藻類;弧菌;抑制
algae;Vibrio;inhibitory
Date: 2004
Issue Date: 2011-06-30T08:40:27Z
Abstract: 中文摘要 本論文的目的為探討水體中微藻與細菌之間的關係,進而了解微藻抑制病原菌生長之效果。本實驗利用五種海水微藻Skeletonema costatum、Chaetoceros muelleri、Tetraselmis chui、Nannochloropsis oculata與Isochrysis galbana的基礎生物特性:生長階段之指數期和細胞數與吸光值之間的關係,觀察微藻的活藻細胞及細胞內產物在不同濃度的環境中對於抑制病原性弧菌Vibrio alginolyticus生長的效果。 由生長曲線結果可得知,微藻細胞大小與指數生長期之最高細胞數呈現反比關係;此外,五種海水微藻的生長速率順序依次為N. oculata > I. galbana > C. muelleri > S. costatum > T. chui。 以微藻抑制V. alginolyticus生長之研究中,發現五種海水微藻在不同濃度之活藻細胞環境中皆有抑制V. alginolyticus生長之效果,其中以I. galbana、C. muelleri抑制效果最為顯著,可以達到完全抑制的效果;微藻之細胞內產物抑制V. alginolyticus生長之研究中,發現僅有S. costatum在不同濃度環境下皆能夠有效降低V. alginolyticus生長。
Abstract The purpose of this study is investigated the relationship of algae and bacteria in the seawater, and also want to know the effect of microalgae inhibit bacterial or not. Five species of microalgae, Skeletonema costatum, Chaetoceros muelleri, Tetraselmis chui, Nannochloropsis oculata and Isochrysis galbana were used to study the inhibition of Vibrio alginolyticus growth with microalgae live cell and intracellular products in different concentrations, based on biological characteristic (log phase of growth curve and relationship of cell count and absorbance). The size of microalgae showed that a negative correlation with the highest cell count in log phase, growth rate was found highest in N. oculata, follow by I. galbana, C. muelleri, S. costatum and T. chui, respectively. All species were found effectively depressed the growth of V. alginolyticus. Moreover, a completely inhibit of V. alginolyticus was found in I. galbana and C. muelleri. On the other hand, different concentrations of S. costatum can inhibit V. alginolyticus growth, only.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M92330038
http://ntour.ntou.edu.tw/ir/handle/987654321/16611
Appears in Collections:[水產養殖學系] 博碩士論文

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