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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/15787

Title: 鉛對烏魚(Mugil cephalus)不同臟器細胞活性之毒理研究
Toxicity study of lead on the cell activation of Grey mullet(Mugil cephalus)
Authors: Yi-Jun Lin
林怡君
Contributors: NTOU:Department of Environmental Biology and Fisheries Science
國立臺灣海洋大學:環境生物與漁業科學學系
Keywords: ;烏魚;Indo-1
Lead;Grey mullet(Mugil cephalus);Indo-1
Date: 2006
Issue Date: 2011-06-30T08:19:29Z
Abstract: 摘 要 先以PC12細胞建立神經細胞毒性模式,當在不同鈣離子濃度下鉛離子進入PC12細胞株,發現PC12細胞對於鉛離子加入的螢光反應瞬間下降,顯示出PC12細胞對於鉛離子的吸收是即時的。不管細胞外是否有無添加鈣離子,PC12細胞對於鉛離子刺激所引起的螢光消減,亦隨著鉛離子濃度增加而增加,螢光消減最大值(42.09±0.45)出現在以Pb2+ 20μ刺激而細胞外無添加鈣離子的情形下。 在鉛離子對於PC12神經細胞的鈣離子反應之影響中,實驗以ATP(200μM)為刺激藥劑,藉以活化Ca2+-ATPase。細胞外含鉛離子的環境下,細胞內的鈣離子濃度明顯受到抑制與對照組(2756.56±277.40 nM)相差三倍。 再以烏魚苗(平均體長5.04±0.12 cm,平均體重2.44±0.15 g)的鰓、肌肉、肝臟、脾臟及腸道各臟器之解離細胞為實驗對象,在研究中皆可發現烏魚各臟器細胞對於鉛離子加入的螢光反應是瞬間下降,顯示出烏魚各臟器細胞對於鉛離子的吸收是即時的。 各臟器細胞螢光下降比率最大值分別為鰓細胞41.67±0.23%、肌肉細胞23.80±0.43%、肝細胞49.51±0.80%、脾臟細胞40.23±0.63%、腸道細胞37.66±0.57%。在不同鈣離子環境下以不同鉛離子濃度刺激實驗中,螢光消減以肝細胞的螢光消減最多,其次為脾臟及腸道細胞,最少的是肌肉細胞。 關鍵字;鉛、烏魚、Indo-1。
Abstract The cell model of neurotoxicity set up by PC12 cells. The instantaneous drop in the fluorescence responses after added Pb2+ was decreased immediately. This result indicated the contribution of fluorescence quench in PC12 cells under different concentration of Ca2+ and Pb2+. It’s showed the cellular uptake of Pb2+ in real-time. This increased of Indo-1 fluorescence quench with increasing the concentration of Pb2+ occurred in both the extracellular normal Ca2+ and Ca2+ free medium but was most apparent while 20μM Pb2+ was added to PC12 cells in the Ca2+ free medium. In this experiment of the effects on the calcium reaction in PC12 cells by Pb2+. Added ATP(200μM)as a stimulates drug for activates the Ca2+-ATPase. The concentration of calcium reaction in PC12 cell was being inhibited and three times lower than the control(2756.56±40 nM)by obviously, while the Pb2+ added. The digested cell from the gill , muscle , liver , spleen and bowel of Mugil cephalus fry(5.04±0.12 cm of average long, 2.44±0.15 g of average weight)to be experimental subjects. The instantaneous drop in fluorescence after the addition of Pb2+ was always present and indicated the contribution of fluorescence in all organs cells quench under different concentration of Ca2+ by Pb2+. Show that cellular uptake of Pb2+ in real-time. The maximum of Indo-1 fluorescence quench in cells of gill , muscle , liver , spleen and bowel were 41.67±0.23%, 23.80±0.43% , 49.51±0.80% , 40.23±0.63% and 37.66±0.57, respectively. Effects of different contribution of Pb2+ under extracellular different Ca2+ contribution medium, the maximum of Indo-1 fluorescence quench was the liver cell, secondly was spleen and intestinal cell, the least was the muscle cell. Key words: Lead, Grey mullet(Mugil cephalus), Indo-1.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M93310008
http://ntour.ntou.edu.tw/ir/handle/987654321/15787
Appears in Collections:[環境生物與漁業科學學系] 博碩士論文

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