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Please use this identifier to cite or link to this item: http://ntour.ntou.edu.tw:8080/ir/handle/987654321/15492

Title: 紫菜粉末與紫菜酒粕生理活性探討
Evaluation on the bioactivity of Porphyra dentate powder and wine spent
Authors: Chia-Ming Chen
陳家明
Contributors: NTOU:Department of Food Science
國立臺灣海洋大學:食品科學系
Keywords: 紫菜;紫菜酒粕
porphyra dentate;porphyra dentate wine spent
Date: 2009
Issue Date: 2011-06-30T08:13:49Z
Abstract: 本研究分別以 99% 與 50% 甲醇、95% 與 50% 乙醇及熱水萃取紫菜粉末及其釀酒後之酒粕,分別探討其抗氧化活性、對人類皮膚纖維母細胞增生能力、膠原蛋白合成能力、抑制酪胺酸酶活性、人類黑色素瘤細胞生長能力,以了解其在化妝品應用之潛力。紫菜酒粕粗蛋白及粗脂肪含量均明顯高於紫菜原料,灰分含量則明顯低於紫菜原料。紫菜酒粕原料之總酚含量相當於 4.55 ± 0.20 mg gallate/g,明顯高於紫菜粉末原料 3.17 ± 0.29 mg gallate/g。紫菜酒粕原料之類黃酮含量相當於 1.52 ± 0.27 mg quercetin/g,明顯高於紫菜粉末原料 0.58 ± 0.30 mg quercetin/g。紫菜酒粕原料之還原力相當於 8.56 ± 0.89 mg ascorbate/g,明顯高於紫菜粉末原料 1.30 ± 0.12 mg ascorbate/g。紫菜酒粕各萃取物之還原力以 50% 甲醇萃取物相當於 21.51 ± 2.23 mg ascorbate/g 最多,明顯高於紫菜粉末 99% 甲醇萃取物 14.60 ± 1.74 mg ascorbate/g。紫菜酒粕原料之清除 DPPH 自由基能力明顯高於紫菜粉末原料 2.14 ± 0.99 mg ascorbate/g,而紫菜粉末及紫菜酒粕之清除 DPPH 自由基能力在各萃取物間則無顯著差異。紫菜酒粕原料之螯合亞鐵離子能力相當於 3.00 ± 0.86 mg EDTA/g,明顯高於紫菜粉末原料 2.57 ± 1.31 mg EDTA/g。紫菜酒粕各萃取物之螯合亞鐵離子能力以 99% 甲醇萃取物相當於 9.14 ± 2.62 mg EDTA/g 最好,但明顯低於紫菜粉末 95% 乙醇萃取物 12.91 ± 7.62 mg EDTA/g。0.5 mg/mL 紫菜粉末 50% 乙醇萃取物可使人類皮膚纖維母細胞 CCD-966SK生長率增加至 175%,使 CCD-966SK 之膠原蛋白分泌量 28.83 ± 4.95 g/mL,明顯高於控制組 5.00 ± 4.58 g/mL。紫菜粉末 95% 乙醇萃取物與紫菜酒粕99% 甲醇萃取物、50% 乙醇萃取物與 CCD-966SK培養 24 小時後,細胞生長率較培養 48 及 72 小時高。10 mg/mL 之紫菜酒粕 50% 乙醇萃取物抑制酪胺酸酶活性為 33.94 ± 2.19%,明顯高於紫菜粉末之 25.94 ± 5.02%。紫菜酒粕 99% 甲醇及 95% 乙醇萃取物抑制人類黑色素瘤細胞生長所需濃度 (IC50)分別為 23.33 與 26.83g/mL,低於紫菜粉末 99% 甲醇及 95% 乙醇萃取物所得 IC50 (60.41 及 86.66 g/mL)。紫菜粉末及紫菜酒粕之 99% 甲醇萃取物及 95% 乙醇萃取物可能經由促使人類黑色素瘤細胞 A375 胞內活性氧物質產生而導致細胞死亡。
The aim of this study is to evaluate anti-oxidant capability, proliferation on human skin fibroblast CCD-966SK, collagen secretion capability, inhibition of tyrosinase and human malignant melanoma A375 growth by Porphyra dentate powder and its wine spent extract, which were extracted with 99% and 50% methanol, 95% and 50% ethanol and hot water, to understand its potency on cosmetics. Crdue protein and crude fat of P. dentate wine spent are clearly more than P. dentate powder. Ash of P. dentate wine spent is clearly less than P. dentate powder. Total phenolics of P. dentate wine spent is equivalent to 4.55 ± 0.20 mg gallate/g, is clearly higher than P. dentate powder, which is equivalent to 3.17 ± 0.29 mg gallate/g. Total flavonoids of P. dentate wine spent is equivalent to 1.52 ± 0.27 mg quercetin/g, is obviously higher than P. dentate powder, which is equivalent to 0.58 ± 0.30 mg quercetin/g. Reducing capability of P. dentate wine spent is equivalent to 8.56 ± 0.89 mg ascorbate/g, is clearly higher than P. dentate, which is equivalent to 1.30 ± 0.12 mg ascorbate/g. Reducing capability of 50% methanol extract of P. dentate wine spent is equivalent to 21.51 ± 2.23 mg ascorbate/g, is obviously higher than 99% methanol extract of P. dentate powder, which is equivalent to 14.60 ± 1.74 mg ascorbate/g. Scavenging effect of DPPH radical on P. dentate wine spent is clearly higher than P. dentate powder, which is equivalent to 2.14 ± 0.99 mg ascorbate/g, and there is no significant difference on each extract of P. dentate powder and P. dentate wine spent on scavenging DPPH radical. Ferrous chelating on P. dentate wine spent is equivalent to 3.00 ± 0.86 mg EDTA/g, is clearly higher than P. dentate powder, which is equivalent to 2.57 ± 1.31 mg EDTA/g. Ferrous chelating of 99% methanol extract of P. dentate wine spent is equivalent to 9.14 ± 2.62 mg EDTA/g, is obviously less than 95% ethanol extract of P. dentate powder, which is 12.91 ± 7.62 mg EDTA/g. 50% ethanol extract of P. dentate powder on 0.5 mg/mL promote cell viability of human skin fibroblast CCD-966SK up to 175%. And it enable collagen secretion of CCD-966SK from 5.00±4.58 g/mL up to 28.83 ± 4.95 g/mL. Cell viability of CCD-966SK treated with 95% ethanol extract of P. dentate powder and 99% methanol extract and 50% ethanol extract of P. dentate wine spent for 24 hours is higher than 48 and 72 hours. Tyrosinase inhibition effect on 50% ethanol extract of P. dentate wine spent on 10 mg/mL is 33.94 ± 2.19%, which is clearly higher than P. dentate powder, which is 25.94 ± 5.02%. Concentration of 50% inhibition (IC50) in human skin malignant melanoma A375 on 99% methanol extract and 95% ethanol extract of P. dentate wine spent are 23.33 and 26.83g/mL, which are lower than 99% methanol extract and 95% ethanol extract of P. dentate powder, which are 60.41 and 86.66 g/mL. 99% methanol extract and 95% ethanol extract of P. dentate powder and P. dentate wine spent may induces A375 cell death by generating intracellular reactive oxygen species.
URI: http://ethesys.lib.ntou.edu.tw/cdrfb3/record/#G0M96320049
http://ntour.ntou.edu.tw/ir/handle/987654321/15492
Appears in Collections:[食品科學系] 博碩士論文

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